Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Hersteller:
Humana Press in Springer Science + Business Media
juergen.hartmann@springer.com
Heidelberger Platz 3
DE 14197 Berlin

Contents Preface Contributors PART I INTRODUCTION 1. Definitions and Criteria for Stem Cells Leslie P. Weiner 2. Neural Stem Cells and the Future Treatment of Neurologic Diseases: Raising the Standard Jaime Imitola and Samia J. Khoury PART II ISOLATION AND CULTURE OF NSCS 3. Neural Differentiation of Human Embryonic Stem Cells Mirella Dottori and Martin F. Pera 4. Generating Neurons from Stem Cells Andreas Androutsellis-Theotokis, Sachiko Murase, Justin D. Boyd, Deric M. Park, Daniel J. Hoeppner, Rea Ravin, and Ron McKay 5. Isolation of Neural Stem and Precursor Cells from Rodent Tissue Yuanyuan Wu, Ying Liu, Jonathan D. Chesnut, and Mahendra S. Rao 6. Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization Lamya S. Shihabuddin 7. Adult Neural Stem Cells Rossella Galli, Angela Gritti, and Angelo L. Vescovi 8. Olfactory Ensheathing Cells: Isolation and Culture from the Olfactory Bulb and Olfactory Epithelium Susan C. Barnett and Jane Roskams 9. Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property Seung U. Kim, Atsushi Nagai, Eiji Nakagawa, Hyun B. Choi, Jung H. Bang, Hong J. Lee, Myung A. Lee, Yong B. Lee, and In H. Park 10. Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood Shijie Song and J. Sanchez-Ramos 11. Production of Neurospheres from CNS Tissue Gregory P. Marshall II, Oleg Suslov, Tong Zheng, Dennis A. Steindler, and Eric D. Laywell 12. Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda, and Kazunari Yokoyama PART III IN VITRO STUDIES A. CELLULAR TECHNIQUES 13. Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures Angela Gritti, Rosella Galli and Angelo L. Vescovi 14. Telomere Neurobiology Mark P. Mattson, Peisu Zhang and Aiwu Cheng 15. Chromosomal Mosaicism in Neural Stem Cells Suzanne E. Peterson, Jurjen W. Westra, Christine M. Paczkowski, and Jerold Chun 16. In vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype Marcel M. Daadi 17. In vitro Migration Assays of Neural Stem Cells Pascale Durbec, Isabelle Franceschini, Francoise Lazarini, and Monique Dubois-Dalcq 18. Measuring Apoptosis in Neural Stem Cells Brett T. Lund and Eve E. Kelland 19. Markers of Adult Neural Stem Cells Eric Wexler B.MOLECULAR TECHNIQUES 20. Differential Gene Expression in ES Derived Neural Stem Cells Using RT PCR Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch 21. Microfluidic Devices for High Throughput Gene Expression Profiling Single hESC-derived Neural Stem Cell Yan Chen and Jiang F. Zhong 22. Electroporation Strategies for Genetic Manipulation and Cell Labeling Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark 23. Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches Luciano Conti and Elena Cattaneo PART IV STUDIES FOR TRANSPLANTATION 24. Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo Sanjay S. Magavi and Jeffrey D. Macklis 25. Immunocytochemical Analysis of Neuronal Differentiation Sanjay S. Magavi and Jeffrey D. Macklis 26. Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold < ...