Public Health Microbiology: Methods and Protocols is focused on microorganisms that can present a hazard to human health in the course of everyday life. There are chapters dealing with organisms that are directly pathogenic to humans, including bacteria, viruses, and fungi; on organisms that produce toxins during growth in their natural habitats; on the use of bacteriocins produced by such organisms as lactobacilli and bifidobacteria; as well as several chapters on hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, and microbiological tests for sanitation equipment in food factories. Additional chapters look at the use of animals (mice) in the study of the various characteristics of milk and their relationships with lactic acid bacteria in particular. Other chapters focus on special methods for determining particular components of milk. In particular, in Parts I and II, on bacterial and viral pathogens, special attention is given to methods for PCR detection of genes with resistance to tetracycline, as well as to Salmonella enterica; for identification and typing of Campylobacter coli; for detection of the abundance of enteric viruses, hepatitis A virus, and rotaviruses in sewage, and of bacteriophages infecting the O157:H7 strain of Escherichia coli. Part III offers methods for computerized analysis and typing of fungal isolates, for isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone.

Hersteller:
Humana Press in Springer Science + Business Media
juergen.hartmann@springer.com
Heidelberger Platz 3
DE 14197 Berlin

Part I. Bacteria Detection of Tetracycline Resistance Genes by PCR Methods Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, and Roderick I. Mackie Integron Analysis and Genetic Mapping of Antimicrobial Resistance Genes in Salmonella enterica serotype Typhimurium Mairead Daly and Seamus Fanning Molecular-Based Identification and Typing of Campylobacter jejuni and C. coli Brigid Lucey, Fiona O'Halloran, and Seamus Fanning Molecular Genotyping Methods and Computerized Analysis for the Study of Salmonella enterica Ana Belen Vivanco, Juan Alvarez, Idoia Laconcha, Nuria Lopez-Molina, Aitor Rementeria, and Javier Garaizar Part II. Viruses Detection of Infectious Rotaviruses by Flow Cytometry Albert Bosch, Rosa M. Pinto, Jaume Comas, and Francesc-Xavier Abad Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples Kelly A. Reynolds Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7 Maite Muniesa and Juan Jofre Molecular Genotyping of Irish Rotavirus Strains Fiona O'Halloran and Seamus Fanning Hepatitis A Virus: Molecular Detection and Typing Glòria Sanchez, Cristina Villena, Albert Bosch, and Rosa M. Pinto Part III. Fungi Typing Fungal Isolates: Molecular Methods and Computerized Analysis Aitor Rementeria, Ana Belen Vivanco, Ainara Cadaval, María Teresa Ruesga, Sonia Brena, Jose Ponton, Guillermo Quindos, and Javier Garaizar Fungal Isolation and Enumeration in Foods Dante Javier Bueno, Julio Oscar Silva, and Guillermo Oliver Determination of Aflatoxins and Zearalenone in Different Culture Media Dante Javier Bueno and Guillermo Oliver Part IV. Other Pathogens Intracellular Multiplication of Legionella Species and the Influence of Amoebae on Their Intracellular Growth in Human Monocytes: Mono Mac 6 Cells and Acanthamoeba castellanii as Suitable In Vitro Models Birgid Neumeister Viability of Amoebae, Fungal Conidia, and Yeasts: Rapid Assessment by Flow Cytometry Judith A. Noble-Wang, Shangtong Zhang, Daniel Price, and Donald G. Ahearn Detection and Differentiation of Cryptosporidium Oocysts in Water by PCR-RFLP Lihua Xiao, Altaf A. Lal, and Jianlin Jiang Genotyping of Cryptosporidium parvum With Microsatellite Markers Giovanni Widmer, Xiaochuan Feng, and Sultan Tanriverdi Immunomagnetic Separation of Pathogenic Organisms From Environmental Matrices Gary P. Yakub and Kathleen L. Stadterman-Knauer Detection of Erysipelothrix rhusiopathiae in Clinical and Environmental Samples Silvana G. Fidalgo and Thomas V. Riley Interaction Between Lactic Acid Bacteria and Gastrointestinal Nematodes of Caprine Origin Diana Draksler, María Cecilia Monferran, and Silvia Gonzalez Molecular Detection of Genes Responsible for Cyanobacterial Toxin Production in the Genera Microcystis, Nodularia, and Cylindrospermopsis Brendan P. Burns, Martin L. Saker, Michelle C. Moffitt, and Brett A. Neilan Part V. Bacteriocins and Other Inhibitors Purification of Antilisterial Bacteriocins Jean-Marc Berjeaud and Yves Cenatiempo The Hazard Analysis and Critical Control Point System in Food Safety Anavella Gaitan Herrera Testing Disinfectants in the Food Factory: Phenol Coefficient Method Anavella Gaitan Herrera Nontraditional Method of Evaluating Disinfectants: With Isolated Microorganisms From the Food Factory Anavella Gaitan Herrera Microbiological Analysis of Cosmetics Anavella G ...